Objective To establish a method which utilize the new europium fluorescent chelate BHHCT as label for highl sensitive detection of hepatitis C virus.Methods HCV RNA extracted by chromatography was one-round amplified by Tth enzyme PT-PCR in which,the primers were pre-labeled with biotin.The amplified products were hybridized with capture probe pre-fixed on the microplate.By conjugation of biotin with europium-labeDNAled streptavidin,Europium was linked to the target DNA,and fluorescent of europium was measured.Results The CV values,sensitivity and specifity of this method were 10.43%,100%,100% respectively,compared with enzyme-labeled assay 16.25%,94%,100%,Conclusion Europium labelled RT-PCR assay is a sensitive,specific,fast and precise method for detection of hepatitis C virus.