Objective To explore a new method to standardize the sensitivity test for antifungal drug to dermatophytes.Methods A reverse diffusion susceptibility test was established.The new method based on the dilution of antifungal drug in solid medium.The agar with colonies of trichophyton mentagrophytes and microsporum canis was cut by standardized borer from the plates and inoculated into the medium with itraconazole.Regression was used to analyse the supressive rate.Results The higher the concentration of drug was,the smaller colony was.There was positive relation between the area of inocula and the diameter of colony,but there was no relation between the cut part and the diameter of colony.Conclusion The method in this study is reliable,fast and reproducible.