Objective To Survey the resistance of Staphylococcus aureus to vancomycin and genotype Staphylococcus aureu with decreased vancomycin-susceptibility.Methods One hundred and thirty-two strains of Staphylococcus aureu were investigated by growing test on brain-heart infusion (BHI) medium containing 2 microgram/mL and 4 microgram/mL of vancomycin respectively.The stains which grew on BHI medium containing 4 microgramme/mL of vancomycin were genotyped by arbitrarily primed polymerase chain reaction (AP-PCR).Heterogeneous vancomycin-resistant Staphylococcus aureus (hetero-VRSA) isolate was screened and identified by bacterial population analysis method.Results Among the 132 clinical isolates,62(47.0%)stains grew on BHI medium containing 2 microgram/mL of vancomycin.Nine (6.8%) stains grew on BHI medium containing 4 microgram/mL of vancomycin and showed stable and distinct AP-PCR pattern,but the MIC of vancomycin for all the 9 strains were less than 2 microgram/ml.One strain with hetero-VRSA was isolated and identified.Conclusion None of Staphylococcus aureus isolates was confirmed as vancomycin-complete resistance or vancomycin-intermediate resistance.The percentage of hetero-VRSA was 0.75%.There was no prevalent tendency of decreased susceptibility of Staphylococcus aureu to vancomycin.