Objective To prepare monoclonal antibodies (McAbs) against oxidized low density lipoprotein (Ox-LDL). Methods Balb/c mice were immunized with oxidized low density lipoprotein and the hybridoma cell lines secreting monoclonal antibodies against Ox-LDL were established using routine hybridoma technique. The ascites induced were purified with protein A affinity chromatography. The McAbs were analyzed by ELISA and Western blotting to test their biological activities. Results Two hybridoma cell lines,named 6E9D and 7G3C,were developed, which could secret Ox-LDL McAbs stably. Both of them belonged to IgG1 subclass. The numbers of hybridoma chromosomes were 100 and 106. The titers of ascites were 1 :105. Two hybridoma cell lines secreting McAbs against Ox-LDL with high specificity were identified by Western blotting and ELISA. Conclusion The monoclonal antibodies against Ox-LDL with high activity and specificity was established successfully, which could provide a potential value for establishing a new method to measure the serum concentrations of Ox-LDL.