Objective To research the effective method of antigen transfection into dendritic cells(DC).Methods Human dendritic cells were derived from peripheral blood monocytes.The plasmid pEGFP-N1 was transfected into DC with liposome or by Nucleofector Kit.The transfecting efficiency was detected by microscopy and FCM.Results The efficiency of Nucleofector Kit was significantly higher than that of liposome.No significant differences of the cellular morphology and surface molecules expression level of DC were shown between the transfections by liposome and Nucleofector Kit.Conclusion Nucleofector Kit is an effective method for antigen transfection and may provide a new strategy of tansfection for antigen-modified DC vaccine.