Objective To investigate the significance of detection for FP248 mRNA in leukemia and solid tumors.Methods RT-PCR and real-time PCR were used to analyze the expression level of FP248 mRNA in the tumor cell lines, i.e., SGC, A549, D95, SW480, U937, PC3, the bone marrow, and peripheral blood from the patients with leukemia, i.e., ALL, CML, AML, and the cancer tissues in alimentary tract. FP 248 mRNA in peripheral blood mononuclear cells (PBMC) of the patients with alimentary tract cancer was also detected by RT-PCR.Results FP248 mRNA was strongly expressed in SGC but weakly expressed in A549 and D95 cells., No expression was detected in the other tumor cell lines. The expression of FP248 mRNA was up-regulated in the bone marrow and peripheral blood of the patients with ALL, AML, CML and the tissues of stomach cancer, colon cancer and rectal cancer comparing with control groups. No FP248 mRNA was detected in esophageal cancer tissue and PBMC of the patients with alimentary tract cancer.Conclusions FP248 gene might play an important role in nosogenesis and development of ALL, CML, AML, stomach, colon and rectal cancer, so it should be a useful marker for diagnosis of leukemia and alimentary tumors.