Abstract: Objective：To evaluate the accuracy of measurement values for α-amylase in serum by routine methods. Methods：Four commercial reagents for α-amylase were selected to establish 11 α-amylase routine methods in Hitachi 7170 chemical analyzer including 4 approved test systems (A1, B1, C1 and D1) and 7 conventional methods (A2, B2, C2, D2, B3, C3 and D3). The levels of α-amylase in frozen human serum samples covering the range from 40 to 750 U/L were measured simultaneously by using IFCC reference method and 11 routine methods. The accuracy of measurement was evaluated according to the guideline of EP9-A2. The consistency of the results between each routine method and IFCC reference method was analyzed by the improved BlandAltman semi-graphical method. The accuracies of the measurement results of each routine method were evaluated comprehensively. Results：The regression equations of the measurement values between each routine method and IFCC reference method were Y=0.934X＋0.886, Y=1.070X－6.249,Y=0.930X＋5.388, Y=0.731X－3.574, Y=0.986X＋0.996, Y=0.983X－6.195, Y=0.971X＋6.957, Y=1.000X－4.649, Y=0.715X－4.648, Y=0.864X＋4.821 and Y =0.757X－4.253, respectively. In the equations, X stood for the measurement values of IFCC reference method and Y stood for the measurement values of each routine methods. Eleven routine methods were evaluated by using IFCC reference method as the comparative method. The measurement results of the 4 methods, i.e., B1, A2, C2 and D2 were reliable. Conclusions：Using frozen human serum samples as calibrators should be an excellent solution for the accuracy of α-amylase values measured by routine methods.