Abstract: Objective：To prepare recombinant fructosebisphosphate aldolase (Fba1) of Candida albicans and identify its immunogenicity. Methods：The full length coding sequence of Fba1 was obtained by PCR from genomic DNA of C.albicans C1 strain, and cloned into pMD18-T vector. The sequencing demonstrated the amplified DNA sequence of Fba1 to be correct. The Fba1 sequence was connected with the prokaryotic expression vector pET28a(+) to construct the recombinant expression vactor pET28a(+)/Fba1 which was then transferred into E.coli BL21(DE3) and the recombinant fusion protein His6-Fba1 was induced with IPTG. The His6-Fab1 was further purified with TALON Metal Affinity Resin, and identified by Western blot using the monoclonal antibody against His6-tag. The immunogenicity of His6-Fab1 was confirmed by western blot with the sera from the patients with systemic candidiasis. Results：The sequence of prepared fulllength DNA of C.albicans Fba1 was coincident with that registered in GenBank. The recombinant protein His6-Fba1 was highly expressed in E.coli with final yield of 7.6 mg/g in soluble form. Western blot showed that recombinant His6-Fba1 protein could specifically react with monoclonal anti-his-tag antibody and the anti-enolase antibody in the sera from candidiasis patients. Conclusion：The recombinant fusion protein His6Fba1 with high immunogenicity was successfully prepared, and it should be helpful to develop a novel specific assay for diagnosis of invasive candidiasis.