Abstract：Objective：To study the correlation between infection of human papillomavirus (HPV) and distribution of HPV genotype in male patients. Methods：The swab samples of 357 male patients aged from 16 to 68 years old were collected. Twenty-one genotypes of HPV were detected by PCR and reverse line blot hybridisation. The correlation between HPV genotypes and clinical symptoms was analyzed. Results：The total positive rate of HPV infection was 43.7% (156/357) in the tested male patients. Among them, the positive rate of HPV infection in the samples from glans/corona and penis was 44.9% (136/303). The prevalence of excrescence, papule, erythema and asymptomatic infection was 81.0%, 53.1%, 40.0% and 20.5% respectively, and significant differences of HPV infection among the different symptom groups (P<0.05) were found. The five patients with bowenoid papulosis were all infected by high risk HPV. The positive rate of HPV in the patients with condyloma acuminate, who were genotype of moderate or low risk HPV, was 93.6% (73/78). All of 21 HPV genotypes were detectable. The genotypes with high prevalence successively were HPV6 (22.1%), HPV11 (21.7%), HPV16 (11.2%), HPV52 (5.6%), HPV18 (4.8%) and HPV58 (4.0%). Conclusion：The asymptomatic infection of HPV was common in the men attending venereal disease clinics. HPV genotypes may associate with the symptoms of HPV infection.