Abstract: Objective:To investigate the allele frequencies of human platelet alloantigens (HPA) in Han population of Henan province, and establish the gene bank of platelet antigens for local population in order to provide experimental evidence for compatible platelet transfusion. Methods:The HPA1 to 16bw of 160 unrelated and healthy Henan subjects were genotyped by polymerase chain reaction with sequencespecific primer (PCR-SSP). Results:The frequencies of HPA-1 to 6 and HPA-15 were as follows：0.987 5 and 0.012 5 for HPA-1aand 1b, 0.946 9 and 0.053 1 for HPA-2a and 2b, 0.590 6 and 0.409 4 for HPA-3aand 3b, 0.996 9 and 0.003 1 forHPA-4a and 4b, 0.990 6 and 0.009 4 forHPA-5aand5b, 0.981 2 and 0.018 8 for HPA-6a and6b, 0.540 6 and 0.459 4 forHPA-15a and 15b. Only a/a homozygosity of HPA7-14bw and HPA-16bw were detectable and both the gene frequencies was 1.000 0. The detected HPA gene frequencies corresponded to Hardy-Weinberg equilibrium. The distribution of HPAgene showed ethnic and regional diversity. Conclusion:The heterozygous rate of HPA-15and HPA-3 were the highest in Han population of Henan province. HPA genotyping could conduce to reduce the incidence of immune thrombocytopenia during transfusion of platelet.