Abstract: Objective：To explore the effects of coagulation factor Ⅶa on the proliferation of colon cancer cells SW620 and the expression of apoptotic molecule caspase-3, as well as the mechanisms involved in the process. Methods：SW620 cells were treated with protease activated receptor-2 agonists (PAR2-AP) and coagulation factor Ⅶa respectively, then the proliferation rate of the cell was observed. Meanwhile, the expression levels of caspase-3 protein and mRNA in SW620 cells were detected by western blot and quantitative real-time PCR (QT-PCR), respectively. The specific antibody to tissue factor (а-TF), antagonist for PAR2 (PAR2-аAP) and inhibitors for ERK1/2 (U0126), NF-κB (PDTC) and p38MAPK (SB203580) were used to investigate the effects of factor Ⅶa on the expression of caspase-3. Results：After the treatment of PAR2-AP (100 μmol/L) and factor Ⅶa (10 nmol/L), the growth of SW620 cells was promoted significantly and the expression of caspase-3 protein and mRNA in the cells was downregulated. The inhibitory effect of factor Ⅶa on the expression of caspase-3 in SW620 cells was obviously reversed by a-TF, PAR2-aAP, U0126 and PDTC, but not by SB203580. Conclusion：Factor Ⅶa inhibited the expression of caspase-3 in SW620 cells, then promoted the proliferation and growth of SW620 cells via TFactivated PAR2 and ERK1/2/NF-κB signal pathway.