Abstract: Objective：To investigate the impact of miR-544a on the invasion metastasis of lung cancer cells and its molecular mechanism. Methods：miRNA expressions of non small cell lung cancer (NSCLC) cell lines, 95C and 95D, were analyzed by miRNA microarray, and the expression level of miR-544a was further determined by real-time quantitative PCR. The target genes of miR-544a were predicted by the Targetscan software. Then, the invasion and metastasis ability of 95C and 95D cells was detected by the Transwell test, and the expression of the target genes by western blot. Results：The level of miR-544a expression in 95D cells was significantly higher than that in 95C cells (2.95±0.26 vs 1.00±0.00, t=12.94, P<0.05). After 95C cells were transfected with miR 544a mimic, the number of cells was increased significantly (32.00±1.00 vs 13.33±0.01, q=18.67, P<0.01), and the expression of E cadherin (CDH1) down regulated (0.202±0.017 vs 0.841±0.052, q=63.86, P<0.01) and that of vimentin up-regulated (0.574±0.009 vs 0.479±0.011, q=9.45, P<0.01). After 95D cells were transfected with miR-544a inhibitor, the number of cells was decreased obviously (11.00±1.00 vs 24.67±0.58, q=13.67, P<0.01), and the expression of CDH1 up regulated (0.769±0.014 vs 0.583±0.010, q=18.67, P<0.01) and that of vimentin down-regulated (0.320±0.020 vs 0.919±0.019, q=5.99, P<0.01). Targetscan predicted that CDH1 may be the target gene of miR-544a. Conclusion：miR-544a promotes the invasion and metastasis of lung cancer cells probably by the down regulation of CDH1 and the up-regulation of vimentin.