Abstract：Objective：To investgate the biocompatibility between chitosan scaffold (CS) and cells in vitro to provide data for the application of CSin vivo. Methods：The effects of CS for the biological characteristics of human gastric adenocarcinoma cell line BGC-823 were evaluated with cell morphological examination and MTT assay. The cells were co-cultured in the leaching solution containing gelatin-immobilized CS with a series of concentrations. The morphological changes of the cells were observed under invert microscope per 24 hour and the cell counting was conducted to plot the growth curve. The effects of CS on the relative proliferation rate of BGC-823 cells were determined by MTT assay. Results：No remarkable morphological change in the cells was found during the culture with CS solution in vitro. The leaching solutions of CS showed no significant inhibition on either growth or proliferation of the cells (P＞0.05). The cytotoxicity gradation of various concentrations of the leaching solution of CS was ranged from 0 to1 degree. Conclusion：CS showed high biocompatibility with BGC-823 cells and may become valuable medical biomaterials.