Abstract: Objective: To evaluate the application of flexible multi-analyte profiling technology (xMAP) and flow-through hybridization and gene chip (HybriMax) in the detection of human papillomavirus (HPV) subtypes. Methods：According to the Clinical and Laboratory Standards Institute (CLSI) EP12-A2 document, the C50 values of xMAP and HybriMax were compared and their detection sensitivity was analyzed. Cervical exfoliated cell samples from 751 women with cervical lesions were detected by the xMAP method, and the obtained results were used to analyze the clinical coincidence. In addition, the HPV subtypes of 535 specimens with abnormal thinprep cytologic test (TCT) results were determined by xMAP and HybriMax technology, respectively, and the consistency of high risk types of HPV (HR-HPV) was compared. Results：The C50 value for xMAP was less than that for HybriMax. The HPV infection rates increased with the advance of cervical lesions including subclinical HPV infections (SPI), cervical intraepithelial neoplasia (CIN) Ⅰ, CIN Ⅱ and CIN Ⅲ (χ²=143.15, P<0.01). The Kappa value reflecting the consistency between xMAP and HybriMax was 0.76. There was no any difference in the constituent ratio of 13 HR-HPV subtypes between xMAP and HybriMax (P>0.05). Conclusion: There was a good consistency in the detection of HPV subtypes between xMAP and HybriMax. Moreover, the xMAP technology was more sensitivity and easy operation than the HybriMax, and was suited to screen and monitor the HPV infection of high risk population with cervical diseases.