Abstract: Objective：To investigate the drug resistant genes and molecular epidemiology of vancomycin-resistant Enterococcus (VRE) isolated from our hospital. Methods：Seven strains of VRE were isolated during 2012 to 2013, and validated by E-test. Then, their genomic DNA were extracted, and the Van type and the genes encoding virulence factors such as asal, gelE, cylA, esp and hyl were detected by PCR and multiplex PCR, respectively. The molecular epidemiology of VRE strains was investigated by multilocus sequence typing (MLST) and pulsed field gel electrophoresis (PFGE). Results：All the seven VRE strains were vanA type and six of them were positive for esp and hyl genes. Both PFGE and MLST showed that stains 2, 4, 5 and 7 were proved to be the dominating type ST 17, and that the other three strains possesed different types. Conclusion：All VRE isolates in our hospital were vanA type and most had virulence genes esp and hyl. MLST and PFGE showed that there was a small scale of VRE prevalence in our hospital, indicating that more attention should be paid to monitor the VRE.