Abstract: Objective：To establish the real time PCR methods for detecting the mRNA levels of insulin-like growth factor-1 (IGF-1), IGF-1 receptor (IGF-1R), IGF-2 and IGF-2 receptor (IGF-2R). Methods：The primers and TaqMan probes for IGF-1, IGF-1R, IGF-2 and IGF-2R were designed and synthesized. Then, the total RNA from placenta tissues was extracted, reversely transcript to cDNA and amplified by PCR with the specific primers for IGF-1, IGF-1R, IGF-2 and IGF-2R. The obtained PCR products were purified and connected with the plasmid vector to construct the recombinant plasmid, which was used as the standard of the established real time PCR. Next, the IGF-1, IGF-1R, IGF-2 and IGF-2R genes were detected by the established real time PCR and verified by DNA sequencing. Results: The obtained PCR products were confirmed as the specific fragments of IGF-1, IGF-1R, IGF-2 and IGF-2R by DNA sequencing, respectively. The linear range, the inter assay coefficient of variation, the intra assay coefficient of variation, the correlation coefficient and the amplification efficiency were 1.00×10²~1.00×10⁸ copies/μL, 1.64%~2.83%, 1.36%, 0.994 and 92.35% for IGF-1, 2.00×10²~2.00×10⁸ copies/μL, 1.73%~1.98%, 1.02%, 0.993 and 96.06% for IGF-1R, 3.00×10²~3.00×10⁸ copies/μL, 2.47%~4.09%, 1.04%, 0.995 and 94.92% for IGF-2, and 5.00×10²~5.00×10⁸ copies/μL, 2.25%~2.52%, 0.96%, 0.996 and 98.84% for IGF-2, respectively. Conclusion :The real time PCR methods with high sensitivity and specificity for detecting the mRNA levels of IGF-1, IGF-1R, IGF-2 and IGF-2R were established successfully.