Abstract: Objective：To investigate the applicable value of DNA microarray technology in detecting CYP2C19 gene polymorphism. Methods：Polymerase chain reaction (PCR) combined with DNA microarray technology was used to detect CYP2C19 genotype of 300 patients with mental disorder. DNA sequencing was carried out to verify the reliability of the results detected by microarray technology. Results：The genotypes of extensive metabolizer , moderate metabolizer and poor metabolizer were detectable in 109 (36.3%),158 (52.7%) and 33 cases (11.0%) respectively. The results of DNA sequencing were completely consistent with those detected by DNA microarray technology. There was no significant difference of proportion between genotope and phenotype in different genders (all P＞0.05). Conclusion：DNA microarray technology can be used to detect the polymorphism of CYP2C19 gene rapidly and accurately and provide advises based on laboratory examination for personalized therapy instead of DNA sequencing.