Abstract: Objective：To establish a molecular diagnosis method for the tag single nucleotide polymorphisms (SNPs) associated with coronary heart disease (CHD) using high resolution melting (HRM) technology, and discuss the association of serum lipids index and susceptibility of CHD in Han population in Lanzhou. Methods：PCR-HRM assays were performed to detect seven SNPs (rs1205 C>T, rs4305 A>G, rs4353 G>A, rs7830 C>A, rs12528076 C>T, rs939899 A>G and rs1108640 C>G) at five CHD associated genes CRP, ACE, NOS3, ENPP1 and IQSEC1. A case control study was performed in 260 CHD patients and 238 matched healthy controls to verify the availability of the method. Results：All samples were successfully genotyped by high resolution melting curve analysis after PCR amplification and correct rate of 100 percent was verified by direct sequencing. Case control analysis showed that the allele frequencies of rs4353 G>A and rs12528076 C>T in CHD group were significantly different from control groups (χ²=3.998, P=0.045；χ²=3.918，P=0.047), and the frequencies of rs7830 C>A genotype between the two groups were significantly different (χ²=6.369，P=0.041), The analysis of serum lipid levels showed that rs1205 locus in the CHD patients was correlated with high density lipoprotein cholesterol (HDL-C) levels (F=14.067, P=0.001). Conclusion：The PCR-HRM assay should be a rapid and accurate genotyping method. The ACE, NOS3 and ENPP1 genes were associated with the susceptibility of CHD in Han population of Lanzhou. The detection of rs1205 C>T combined with HDL-C level may predict the occurrence of CHD.