Abstract: Objective：To construct the induced pluripotent stem cells (iPSCs) from human peripheral blood mononuclear cells (PBMC) using the non integrating reprogramming technique. Methods：Peripheral blood samples from healthy persons were collected and the PBMCs were isolated. Then, the PBMCs were infected with sendai virus containing four reprogramming factors— OCT3/4, SOX2, KLF4 and c-MYC, and the hPBMC-derived iPSCs were obtained by the culture system with feeder-free condition. The multipotency of the obtained hPBMC-derived iPSCs was verified by the observation of cell morphology, alkaline phosphatase (AP) staining, immunofluorescence staining, and the embryoid body formation and differentiation assay, and its safety was evaluated by the karyotype assay and the detection of sendai virus genomic RNA. Results：iPSC-like colonies emerged at day 16 after the PBMCs were infected with sendai virus. The obtained iPSCs were able to be cultured with a few passages, and were demonstrated to have the multipotency. Moreover, the hPBMC derived iPSCs carried normal karyotype, and no any exogenous genomic RNA from sendai virus was detected in it. Conclusion：A non integrating reprogramming system for hPBMC was successfully constructed, and the iPSCs from hPBMC with multipotency, normal karyotype and the lack of exogenous genes were obtained.