Abstract：Objective：To establish an automatic warning program which accommodates to the test procedures in medical laboratory centerand and to put into operation. Methods：Based on laboratory information management system (LIS), automatic warning program was established, which composed of maintenance of warning rules, feedback of vulnerabilities in rules, solutions of feedback targets and evaluation of ruleeffectiveness. The results of performance were assessed after operation for six months in Guangzhou Kingmed Medical Laboratory Center. Results：An automatic warning program containing 13 kinds of rule templates was successfully established. The multipoint warning program via asynchronous structure for six specialized testing procedures was realized. A total of 1 523 warning rules were included in the rule bank and 24 000 reports were verified on average each day. The average passing rate of reports was 70.2% and the passing rate of single test was 83.9%. The approval time for reports was reduced by 10 to 40 minutes compared with that without using the automatic warning program. The efficiency for report approval was improved by 25 percent. Conclusion：Automatic warning program may ensure the high quality of test reports, ease pressure on staff and improve work efficiency