Abstract: Objective：To analyze the HbA1c results of double heterozygotes of hemoglobin(Hb)NewYork and β-thalassemia detected by five different HbA1c detection systems, and compare the early-warning abilities of erroneous glycosylated hemoglobin results for Hb NewYork and β-thalassemia heterozygotes. Methods：Peripheral blood samples from 40 patients without hemoglobinopathies with different range of HbA1c levels were collected to evaluate the consistency of the detected results. Variant Ⅱ system was used as the reference system which successfully completed NGSP Level Ⅰ laboratory certification. Variant Ⅱ Turbo 2.0（VⅡT）, Capillary 2 Flex Piercing（2FP）, Primus Ultra2(Ultra2)and Roche Modular PPI(PPI)800 were used as the comparative systems. The HbA1c in 2 sera from the patients with compand heterozygotes of Hb NewYork was detected by the above systems. Hemoglobin electrophoresis was performed. Gentypes of αand β-globin genes were analyzed by GAPPCR, hybridization and dideoxy chain termination method. Results：The HbA1c values in nonhemoglobinophthy samples obtained using VⅡT 2.0, Ultra2, C2FP and PPI systems were well correlated with that of VⅡ system. The hemoglobin genotypes of the two cases of compand heterozygotes were aa/aa, βIVS2654/βNewYork and aa/aa, β4142/βNewYork, respectively. The proportions of HbA were all 0 while the proportions of Hb NewYork were 93.5% and 94.0% respectively. The HbA1c results of the two cases detected were 4.3%(23 mmol/mol)and 4.5%(26 mmol/mol)by VⅡ, 4.5%(26 mmol/mol)and 4.6%(27 mmol/mol)by VⅡT 2.0, no values and no values by C2FP, 4.1%(21 mmol/mol)and 4.3%(23 mmol/mol)by Ultra2 and 4.2%(22 mmol/mol)and 4.8%(29 mmol/mol)by PPI systems, respectively. All the systems did not send warning for abnormal signs in profiles and results. Conclusion：The five systems presented different earlywarning ability for the double heterozygotes of Hb NewYork and β-thalassemia. The compand heterozygotes should theoretically not contain HbA1c, but the systems VⅡ, VⅡT, Ultra2 and PPI showed detectable results of HbA1c indicating all the four systems were not able to provide with earlywarning. C2FP system did not report HbA1c result so it exhibited earlywarning ability for the double heterozygote.