Abstract: Objective：To design the ISO15189 management information middleware based on laboratory information management system (LIS) and evaluate its application. Methods：The ISO15189 management information middleware was designed based on LIS and the middleware technology as underlaying platform, and the multifunction management of LIS was implemented by the data warehouse technology and the data cleaning and extraction technology. Results：The designed middleware made LIS implement the functions such as data exchange and transmission, data sharing, and data intelligence processing ISO15189 management information across multiple systems. It constructed a data warehouse by extracting, transforming, and loading useful data from multiple profession systems in laboratories, and flexibly presented data to the users by operating multidimensional data set from various aspects. Conclusion：The ISO15189 management information middleware based on LIS has high value in the management of ISO15189 accredit, which may make the management work more standard, efficiency, and intelligence.