Abstract: Objective: To investigate the serological and molecular identification of 2 rare B(A) blood groups. Methods: The ABO blood groups of 2 samples from blood donors were detected by routine serological method. The genotype features was identified by PCR-sequence specific primer (PCR-SSP) and direct sequence analysis. Results: The serological results for the 2 blood donors showed the characteristics of B(A) phenotype. The sample 1 was genotyped as BO2 subtype by PCR-SSP and direct sequencing showed B alleles in exon 7, presented nt640 A＞G mutation which was confirmed to be B(A)04/O02 genotype. The sample 2 was genotyped as BO1 subtype by PCR-SSP and direct sequencing showed B alleles presented nt700 C＞G mutation in exon 7 which was confirmed to be B(A)02/O01 genotype. Conclusion: The phenotype of the two samples should be B(A) and the genotypes should be rare B(A)04/O02 and B(A)02/O01.