Objective To investigate the current situation of reference intervals of specific proteins in 861 clinical laboratories all over China. Methods The information of reference intervals was collected by external quality assessment software system based on website. Microsoft Excel 2007 and SPSS 17.0 were used to analyze the results of all laboratories. The data reported include upper and lower limits, the sources of reference intervals, verification and grouping rules. Results Laboratories participating in the specific proteins reference interval were 861. The primary source of reference intervals were instructions of instrument and reagent manufactures (75.2%-79.9%). Only 39.6%-47.4% of participants had the verified reference intervals before clinical use. Only 0.3-2.5% laboratories had considered different gender groups when establishing reference intervals. There shown differences for upper and/or lower limits used by more than 50% laboratories compared to them in industry standards, except for upper limits of C4 (54.9%), lower limits of CRP( 93.7%), PA(52.4%) and TRP(53.1%). Conclusion The implementation of industry standards for reference interval in specific proteins was not satisfactory. Only a small part of laboratories using industry standards, of which a number of laboratories didn't verify reference interval before use, so it is very necessary to promote industry standards of reference intervals.