Abstract：Objective?To observe the age-related change trend of total prostate antigen(tPSA), free prostate antigen(fPSA)and their ratio(fPSA/tPSA)of healthy men in Yangzhou, Jiangsu province, and establish a specific reference range for our laboratory.Methods?A total of 3 402 health male examinees were selected in Jiangsu Armed Police Corps Hospital in 2018, and 1 369 samples were finally involved in the quantile regression analysis.The results of tPSA, fPSA and fPSA/tPSA were extracted from electronic medical record mining system.The 5% and 95% quantile regression models of tPSA, fPSA and fPSA/tPSA were fitted by R language package, and the influence of age on the distribution of reference interval was explored.The reference intervals for different age groups were established with 10 years as a subgroup.Results?The results of tPSA, fPSA, fPSA/tPSA showed significant skewness distribution and age dependence.The upper limit(95% quantile)of the reference range of tPSA and fPSA during 30 to 40 years old was the lowest, and then increased, showing the distribution a shape of "U", while the lower limit(5% quantile)of the reference range of fPSA/tPSA showed a decreasing trend overall.Conclusion?The quantile regression model should be suitable for establishing the reference range of PSA and other non-normal distribution test paremeters.The reference range given by reagent specification could be revised or improved by quantile regression model, and the specific reference range of laboratory may be established by age and other influence factors.