Abstract: Objective To verify the consistency of the reference intervals of thyroid hormone estimated in this investigation with the reference interval in the manual of the currently used reagent and industrial standard ( WS/T 404.10- -2022), and select the reference interval of thyroid hormone suitable for the healthy subjects at this hospital. Methods The indirect sampling technique was used to retrospectively analyze the results of thyroid hormone tests of healthy subjects in the Clinical Testing Center of Suzhou Dushu lake Hospital from December 2022 to August 2023. Nonparametric method was used to estimate thyroid hormone P.s-P97.5 reference intervals and 95% confidence intervals of the testing subjects with different genders and ages, and to explore the significant differences in the data distribution among different gender and age groups. The differences were compared with the reference intervals reconmended by the industrial standard ( WS/T 404.10- -2022) and Roche reagent specification. Results There were significant differences in TSH, FT3, FT4 and TT3 between sexes ( P<0.001, Z>Z* ), but there was no significant difference in TT4 between sexes (P=0.998, Z