Abstract: Objective To investigate the relationship between spem DNA fragmentation index ( DFI) ， high reactive oxygen species(ROS) rate , high mitochonlrial membrane potential ( MMP)rate , and progressive motility (PR) in patients with idiopathic astheno-7zoospermia.Methods A total of 250 patients who visited the Male Depatment of Reproductive Medicine Center,Tangdlu Hospital,from December 2022 to November 2023 were emrolled in this studty. The patients were dividel into mild (20%≤PR<32% , n=86) ,moderate ( 10%≤PR<20% , n=67) , and severe (PR<10%, n=47) asthenozospermia groups，and a normal group ( female factorinfetility， normal semen parameters，PR≥32%, n=50) was formed based on their PR values. T'he levels of sperm DFI, high ROSand MMP rates were measured using flow cytometry , and their correlations with PR were analyzed.Results Compared with the nomalgroup , the asthenozoospemia groups showed significantly lower sperm concentration，motility , normal morphology percentage and highMMP rate ( P<0.05 ) , but significantly high DFI and ROS rate (P<0.01 ).The severe asthenozospemia group had significantly highDPFI anl ROS rate andl high MMP rate was lower than that in the mild and moderate groups (P<0.01 ). No significant differences inDFI, high ROS rate and MMP rates were found between the mild and modeate groups (P>0.05). As the severity of asthenozoospemiaincreasedl, DFT and high ROS rate showed an increasing trendl, while high MMP rate decreased.Comelation analysis revealed a signifi-cant positive correlation between PR and high MMP rate (r=0.439,P<0.01 ) and siguificant negative correlations between PR andhigh ROS rate (r=-0.435,P<0.01 ), DFl (r=-0.478, P<0.01 ). ConclusionT'he patients with idiopathic asthenozoospermia ex-hibit altered levels of sperm DFI, high ROS and MMP rates ，which are associated with the severity of asthenozoospermia.Elevated ROslevels may lead to sperm DNA damage and mitochondrial dysfumction，resulting in decreased sperm progressive motlity.