Abstract: Objective To investigalte the characteristics of epitope distribution on HLA clss [ antigen in the regular platelet donorsfrom Nanjing area and establish the HLA epitope database for regular platelet donors.Methods High-resolution HLA typing was per-formed using Sanger method for the blood samples from 649 regular platelet donors in Nanjing area. The polymorphism of HLA antigenepiopes corresponding to the high-resolution HLA typing results was analyzed using the HL.AEplet Regitry website. The frequencies ofallele frequencies ， HILA haplotype , and HLA antigen epitope were calculated by using the direct counting method. Results Among the649regular platelet donors ，38 HLA-A alleles were detectedl, corresponding to 36 HLA-A epitopes， and the higher frequencies were79GT,144K and 138MI. Seventy-thee HLA-B alleles were detected corresponding to 35 HLA-B epitopes ， and the higher frequencieswere 131S,69TNT，and 8ON. Sixty-four HLA class I antigen epitopes were detected,in which the higher frequencies were 79CT,131S, and 144K.Conclusions The distribution of HLA antigen epitopes in the regular platelet donors of Nanjig area exhibited u-nique polymorphic characteristics. The epiope matching strategies should be established based on the distrilution characteristics ofHLA anigen epiopes ， which may expand the range of available donors and reduce the incidence of platelet transfusion refractoriness.