Abstract : Objective To investigate the application value of the fluorescence quantitative PCR (PCR) meling curve method in thedetection of clarithromycin ( 235 rRNA) and quinolone (gyrA) resistant genes of HIelicobacter pylori (Hp)in fecal samples.MethodsA total of 1 176 untreated patients who underwent gastroscopy and were Hp positive poved by rapid urease test ( RUT) were enrolledin the study. Their gastric mucosal and fecal samples were collectedl. The E-test method was used to analyze the clarithromycin andquinolone resistant phenolypes of Hp in gastric mucosal samples. The qPCR meling curve method was used to delect the clarithromycinand quinolone resistant genotypes of Hp in fecal samples. The consistency of the results obtained by the two methods was evaluated bythe Kappa test. In addition, the nucleic acids were extracted from the fecal samples with Hp positive , and the Hp 23S RNA and gyrAresistance mutation genes were detectedl by the qPCR meling curve methodl and Sanger sequencing, respectively.The consistency of theresulhs obtained by the two methods was compared.Results In the studly of clarithromycin resistance, a total of 934 valid samples wereobtained.Among them , 453 samples had positive resistance phenotype and 481 had positive resistance genotype, with a positive consis-tency rale of 93.38% (95%CI; 90.70%~95.32% ). In the studly of quinolone resistance，a total of 909 valid samples were obtainedl.Among them , 426 samples had positive resistance phenotype and 413 had positive resistance genotype,with a positive consistency rateof 86.85%(95%CI: 83.31%-89.74% ). In the companative studly， 986 valid samples were detected for Hp 23S rRNA gene.Amongthem， 514 samples were resistance positive detected by the qPCR melting curve method and 509 by Sanger sequrencing，with a positiveconsistency rate of 96.27% (959%CI: 94.24%-97.60% ) . Similarly , 895 valid samples were detected for Hlp gyrA gene.Among them,422 samples were resistance positive detected by the qPCR melig curve method and 405 by Sanger sequencing, wih a positive consis-tency rate of 95.80% (95%CI:93.38%-97.36% ) . ConclusionT'he qPCR melting curve mehod can detect Hp 235 rRNA and gyrAin fecal samples ,which has certain clinical application value for predicting the resistance of Hp.