Abstract: ObjectiveTo establish a detection method for common pathogens causing reproductive tract infections using centrifugal mi-crofluidic technology based on the column for nucleic acid extraction in column. MethodsThe specific primer probes were designed and column-based nucleic acid extraction combined qPCRamplification detection were integrated onto a centrifugal microfluidic chip to achieve fully automated detection of 9 common reproductive tract pathogens Mycoplasma hominis，human herpes simplex virus,Chlamydia trachomatis，Ureaplasma urealyticum，Trichomonas vaginalis，Neisseria gonorrhoeae，Herpes simplex virusI,Mycoplasma genitalium，and Treponema pallidum. The minimum detection limit，specificity，repeatability and linearity of this method were evalua- ted, and 255 positive and 45 negative clinical specimens were tested to verify the accuracy. Results The minimum detection limit of the centrifugal microfluidic chip was500 copies/mL. No cross-reactivity with common reproductive tract pathogens was found. The pre-cision was less than 5%，and good linearity was shown（R2>0.99). Compared with the conventional detection methods,the sensitivity,specificity，positivepredictivevaluenegativepredictivevalueandoverallcoincidencerate of thecentrifugalmicrofluidicchipwereall higher than 96.2%. There was no statistically significant difference between the results of the two methods in the detections of 9 common reproductive tract pathogens（P>O.05)，indicating excellent consistency（all Kappa value>0.96).ConclusionThe centrifugal mi-crofluidic technology based on the nucleic acid extraction using the column has shown excellent sensitivity，repeatability，specificity and accuracy for detectingcommonpathogensin reproductivetractinfection,indicating significantclinical application potential.