Abstract: Objective To investigate the antibiotic resistance characteristics and prevalence of biocide resistance gene qacA/B in Staphylococcus aureus（S. aureus) colonized in nasal cavity of the students among primary and secondary schools. MethodsS. aureus strains isolated from 2 372 students of 5 schools（primary,junior high，and high schools)in Guangzhou from October to November 2018 were detected for drug resistance of 16 antibiotics by using Vitek automatic antimicrobial susceptibility test system. Multilocus-se-quence typing(MIST) and PCR detection for qacA/Bgene were also performed. ResultsThe colonization proportion of S.aureus in nasal passages of the students in Guangzhou was 30.43%（722/2 372)，of which methicillin-resistant Staphylococcus aureus（MRSA)accounted for 9.70%（70/722），and high resistance rates to penicillin（85.60%），erythromycin（43.07%）and clindamycin (40.86%)were observed, respectively. The resistances of S. aureus to penicillin, oxacillin,clindamycin, erythromycin and rifampicin in the students of high school were significantly lower than those in primary and junior high school (PO.05). The S.aureus strains carrying qacA/B genes exhibited higher resistance rates to clindamycin(63.01% vs.40.83%）and erythromycin(60.27% vs.38.67%）compared with non-carying strains,and the differences were statistically significant（P