Abstract: ObjectiveTo identify the serological biomarkers associated with liver fibrosis and cirrhosis by analyzing single-cell sequencing data. MethodsThe GSE136103 dataset was selected to analyze the changes in cellular populations in liver tissues of the cirrhosis groupand determine the changes in hepatic stellatecells（HSC).Then,the GSE168933dataset was used to validate them.Concurrent- ly，the signal input and output of HSC cells were detected, and the relative contribution of HSC-specific receptor-ligand pairs was ranked. Based on the top 20 receptor-ligand pairs,the corresponding cytokines were selected.Additionally,the serological markers for 300 patients with liver diseases who visited Ningbo Development Zone Hospital from September 2019 to May 2021 were detected. A Logistic regression diagnostic model was constructed, and its diagnostic value in liver fibrosis and cirhosis was further explored. Results In the GSE136103 and GSE168933 datasets, HSCs were specifically elevated in liver tissues of the patients with cirrhosis. Based on the relative contribution of receptor-ligand pairs，14 cytokines specifically secreted by HSCs,including growth arrestspecific protein 6 （Gas6)，semaphorin3F （Sema3f)，semaphorin3A（Sema3a)，and etc.,were ultimately obtained. The area under the ROC curve （AUC) of these specific cytokines in the diagnosis of liver fibrosis and cirrhosis was O.92. The AUC for the diagnosis of liver fibrosis alone and cirrhosis alone were O.89 and O.88, respectively. While,the AUC of the FIB4 criteria in the diagnosis of liver fibrosis alone and cirhosis alone were both O.86. ConclusionThe HSC-specific cytokines obtained from single-cell sequencing data have god diagnostic value for liver fibrosis and cirhosis,which is superior to the existing FIB-4 criteria.