Abstract: Objective To screen hub genes and signaling pathways associated with acute ischemic stroke （AIS) using bioinformatics methods，identify potential biomarkers，and provide new evidence for the mechanism research of AIS. MethodsThe gene expression dataset GSE37587 of AIS patients and healthy controls was obtained from the public database gene expression omnibus（GEO). The differentially expressed genes（DEGs，log,FC|>1.2,FDR