Abstract: Objective To establish and optimize an automatic review system for eight report-items of infectious disease markers based on fully automated enzyme immunoassay analyzers and improve the review efficiency. MethodsFolowing CISI AUTO-15 and WS/T 616-2018 standards，a six-dimensional rule system was developed by integrating the infinity and Laboratory Information System,includingqualitycontrollinkage，specimenstatusmonitoring,instrumentalarmresponse,dynamic grayzoneoptimization,serologicalpattern interpretation, and historical data comparison. The system underwent three-phase validation(rule establishment: 17 828 samples,rule verification and optimization:6 743 samples,efficacy evaluation: 12 335 samples),with rules and thresholds refined through human-machine comparison. ResultsAn automated review rule system based on the fully automated enzyme immunoassay platform was established. The optimized system achieved sensitivity and specificity of 98.9% and 95.4%，respectively,with positive and negative predictive values of 72.4%and 99.8%,respectively.The autoverification rate was optimized to increase froman initial 77.1% to 85.2，median turnaround time (TAT)was reduced by 23.1% （from 216 minutes to 166 minutes)，and manual review stafing requirements decreased by 5O%. ConclusionThe autoverification system for the eight items of infectious disease panel on automated enzyme immunoassay platfomm employs multi-rule intelligent integration. It is simple, user-friendly， practical, and highly operable，capable of reducing reviewer workload,shortening TAT, and significantly enhancing review efficiency and accuracy.