Abstract: Objective To evaluate the performance and value of the colloidal gold immunochromatographic assay for the rapid detection of carbapenemase types and a part of KPC subtypes in carbapenem-resistant Enterobacterales (CRE). MethodsA total of 128 non-duplicate CRE isolates were collected. Five carbapenemases （KPC,NDM,IMP,VIM, and OXA-48-like)were detected by both col-loidal gold immunochromatography and polymerase chain reaction (PCR). With PCR as the reference method, the accuracy of the col-loidal gold immunochromatographic assay was evaluated. ResultsThe results of PCR showed that lO strains did not produce any of the five targeted carbapenemases,and the remaining 118 strains were carbapenemase producers. Compared with PCR results,the sensitivity of the colloidal gold immunochromatographic assay was 98.3%and the specificity was l0o%. No statistically significant difference was observed in the detection rate between the two methods（P>0.05),with excellent agreement（Kappa=0.90). Notably,the colloidal gold immunochromatographic assay failed to detect the KPC enzymes in CRE strains producing KPC-31 and KPC-33 subtypes. ConclusionColloidalgold immunochromatographic assayis arapid and reliable method for detecting the fivecarbapenemase types in CRE,but it may miss some KPC subtypes,so combination with other detection methods is recommended.